Composite

Part:BBa_K2680280

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)


3G TlpA39

3G TlpA39 is flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

3G TlpA39 is a a temperature sensitive repressor mutated from Tlpa1. Tlpa is a " tunable, orthogonal, temperature-dependent transcriptional repressor providing switch-like control of bacterial gene expression at thresholds spanning the biomedically relevant range of 32-46 °C" 2 Tlpa39 is mutated so that its derepression occurs at a higher temperature, 39 C.

References

1.Telarovic, I. (2017, October 24). Part:BBa_K2500008. Retrieved from https://parts.igem.org/Part:BBa_K2500008

2. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 841
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 1170


[edit]
Categories
Parameters
None